Immunoglobulin carbohydrate requirement for formation of an IgG-IgG complex.

Reaginic Activity Associated with Igg Immunoglobulin

Five human sera with reaginic activity to a number of allergens were fractionated using anion exchange chromatography. In each serum, fractions which contained detectable IgG and no detectable IgA had capacity to fix to skin and subsequently elicit a P-K reaction. Four of these sera had reaginic activity about equally distributed between fractions containing only IgG and fractions containing mi...

The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains

N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific. This situation applies both to humans and mice. The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not ...

ARE IgG-ANTI-FAB2ɤ AND IgG-ANTI-HINGE ANTIBODIES ACTIVE CONSTITUENTS OF INTRAVENOUS IMMUNOGLOBULIN?

Our aim was to find out if immunoglobulins for intravenous use (IVIG) contain IgG-anti-F(ab')2γ (AFab2) and IgG-antiHinge (AH) antibodies (Ab.) and to discuss how AFab2, in particular AH could contribute to immunomodulatory effect of IVIGs. We measured IgG, AFab2 and AH in five IVIG products approved for therapeutic use. IVIG showed higher AFab2 and AH concentration than sera of randomized heal...

Congenital TOXOPLASMOSIS (IgG-IgM) TOXOPLASMA IgG TOXOCARA IgG LEISHMANIA IgG ECHINOCOCCUS IgG CYSTICERCOSIS IgG TRICHINELLA IgG SCHISTOSOMA IgG FASCIOLA IgG HUMAN DIAGNOSTIC Western Blot KITS FOR PARASITOLOGY and MYCOLOGY

The carbohydrate structures of a mouse monoclonal IgG antibody OKT3.

A mouse monoclonal antibody OKT3, of IgG2a isotype, was isolated from hybridoma culture fluid. Sugar analysis showed the presence of sialic acid, galactose, mannose, fucose, and N-acetylglucosamine, i.e. sugars typical for N-glycosidically linked carbohydrate chains. The absence of N-acetylgalactosamine revealed that O-glycosidically linked carbohydrates were not present. The purified antibody ...